Imperial College London
Hard Science

Genetically Engineered Bacteria Let Scientists ‘See’ Into Soil

This is why synthetic biology is astounding.

Charmaine CaparasJuly 20th 2016

Big Brother Is Watching

Scientists can now play Big Brother to microbial communities through new gas biosensors that can “see” into soil. Furthering technological advances in one of humanity’s oldest trades.

Rice University researchers are using genetically engineered bacteria that release methyl halide gases to monitor microbial gene expression in soil samples in the lab. After an engineered bacterium receives genetic information from another bacterium, it releases a gas to “report” the transaction.

Researchers can monitor processes in real time without having to actually see into or disturb a lab soil sample. The new technique can work the same way as fluorescent reporter proteins, which track protein expression and other processes in biological systems.

Image source: Jonathan Silberg and Shelly Cheng/Rice U
Image source: Jonathan Silberg and Shelly Cheng/Rice U

New Frontier For Agriculture

The study describes a new tool to study how microbes trade genetic material in the environment, according to Earth Science professor Caroline Masiello.

She says the process of horizontal gene transfer controls a lot of things that are important to humans either because they’re good—it’s how rhizobia trade the genes they need to fix nitrogen and support plant growth—or they’re bad—it’s how bacteria trade antibiotic resistance in soils.

The new gas biosensors can be used to study nitrogen fixing in agriculture, antibiotic exchange in wastewater treatment, gene transfer in conditions where nutrients are scarce, and the relationship between gene expression in soil and the release of greenhouse gases.

“There are other technologies that will build on this,” says biochemist Jonathan Silberg, an associate professor of biochemistry and cell biology. “The idea of using gases opens up most anything that’s genetically encoded. However, we do need to improve technologies for some of the subtler kinds of questions.”

Scientists expect they will soon be able to test agricultural soil samples to help fine-tune crop growth through more efficient watering and fertilizer use.

 

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