• Shawn Mikula and Winfried Denk have now presented their BROPA method for the staining of a whole brain. The abbreviation BROPA stands for a complex sequence of staining and rinsing steps, including osmium and pyrogallol solutions. The whole staining process takes around four weeks.
  • “Our results show that single axons can be followed reliably and synapses recognized with the serial block face scanning electron microscope in a BROPA stained mouse brain”, says Mikula.
  • As soon as the latest generation of instruments is delivered and tested, which is planned to take over a year, the scientists intend to start the project. “I estimate that just collecting the data will take around two-and-a-half years”, says Denk. In this time, around 40 petabytes of data will be generated. “We will manage the storage somehow, it’s the analysis we’re still worried about”, adds Mikula. The results so

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