- The CRISPR-Cas system has been previously used to insert a foreign DNA sequence into a targeted genomic site in mammalian cells via a process of recombination. Here it was showed that the insertion could be performed using a simplified end joining process.
- The methods used may help investigators genetically engineer cells to produce high levels of certain proteins—for example by placing the DNA sequence of a particular protein at the site of a highly active gene.
- This is useful because it speeds up the discovery and application of the building blocks of genetic engineering. Similar methods are available in simpler organisms already, but mammalian cells are a more stable environment.
Investigators Insert Large DNA Sequence into Mammalian Cells!
7. 13. 15 by Alex Klokus