Using allelic replacement, a pool of targeting oligos is repeatedly introduced into a cell. MAGE can successfully introduce new genetic modifications in about 25% of the cell population, creating billions of variants every 3 hours.
Not only can MAGE simultaneously modify multiple genomic locations across different length scales (i.e., from single nucleotides to whole genes), it is also possible to tune the amount of sequence change per target. This makes it possible to make specific modifications for specific outcomes or to make high-diversity modifications to explore sequence space.
The speed and ease with which MAGE can alter genomes will transform how we approach the manufacturing and production optimization of industrially significant compounds in the bioenergy, pharmaceutical, agricultural, and chemical industries.